Healing effect of andiroba-based emulsion in cutaneous wound healing via modulation of inflammation and transforming growth factor beta 3

Abstract Purpose: To evaluate the effects and mechanisms of andiroba-based emulsion (ABE) topical treatment on full-thickness cutaneous wounds in rats. Methods: The wounds were harvested on days 3, 7, 15, and 20 post-surgery. Wound contraction rate, quantitative immunohistochemistry [macrophages, myofibroblasts, capillaries, collagens (col) I and III, transforming growth factor β3β (TGFβ3)], and tensile strength were assessed. Results: Treated wounds were smaller, contracted earlier and had increased angiogenesis, fewer CD68+ and M2 macrophages on days 7 and 15, but higher on day 20. Myofibroblasts appeared on days 3 to 7 in untreated wounds and on days 7 to 15 in treated wounds. TGFβ3 levels were higher in the treated wounds, less dense collagen fibers, lower col I/III ratios and a higher tensile strength. Conclusion: These results demonstrate the important anti-inflammatory role of treatment and the associated modulation of macrophages, myofibroblasts, and TGFβ3 levels. Collagen fibers in the treated wounds were more organized and less dense, similar to unwounded skin, which likely contributed to the higher tensile strength.

Chemical composition, antimicrobial and antioxidant potential of the essential oil of Guarea kunthiana A. Juss / Composição química, potencial antimicrobiano e antioxidante do óleo essencial de Guarea kunthiana A. Juss

Abstract The essential oils are extracted from plant compounds and can present activities antimicrobial and antioxidant properties. The goals of the present study were: (a) to determine the chemical composition of the essential oil of Guarea kunthiana A. Juss using the method of gas chromatography coupled to mass spectrometry (GC-MS); (b) to evaluate the antimicrobial potential of this oil using the broth microdilution method against different microorganisms: five Gram-negative bacteria, four Gram-positive bacteria and a yeast and (c) to determine the antioxidant activity of the oil using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical assay. The GC-MS analyses allowed identifying 13 constituents, representing 96.52% of the essencial oil composition. The main compounds identified were α-zingiberene (34.48%), β-sesquiphellandrene (22.90%), and α-curcumene (16.17%). With respect to the antimicrobial activity, the essential oil was effective against all the microorganisms tested, except for the bacteria E. coli and K. pneumoniae, which were resistant to the action of the oil. From a general point of view, Gram-positive bacteria were more susceptible to the action of the essential oil than Gram-negative bacteria. The essential oil exhibited antioxidant potential.

Resumo Os óleos essenciais são compostos extraídos de plantas e podem apresentar propriedades antimicrobianas e antioxidantes. O objetivo deste trabalho foi (a) determinar a composição química do óleo essencial de Guarea kunthiana A. Juss pelo método de cromatografia gasosa acoplada à espectrometria de massas (CG-EM); (b) avaliar o potencial antimicrobiano deste óleo pelo método de microdiluição em caldo frente a diferentes micro-organismos, sendo cinco bactérias Gram-negativas, quatro Gram-positivas uma levedura e (c) por fim, determinar a atividade antioxidante do óleo pelo método de captura do radical livre 2,2-difenil-1-picril hidrazil (DPPH). As análises de CG-EM resultaram na identificação de 13 constituintes, representando 96,52% da composição do óleo essencial. Os principais compostos identificados foram α-Zingibereno (34,48%), β-Sesquifelandreno (22,90%) e α-Curcumeno (16,17%). Em relação à atividade antimicrobiana, o óleo essencial foi efetivo frente a todos os micro-organismos testados exceto para as bactérias E. coli e K. pneumoniae, as quais se apresentaram resistentes à ação do óleo. Em geral, as bactérias Gram-positivas foram mais suscetíveis à ação do óleo essencial em relação às Gram-negativas. O óleo essencial apresentou potencial.

Larvicidal efficacies of plants from Midwestern Brazil: melianodiol from Guarea kunthiana as a potential biopesticide against Aedes aegypti

A total of 36 ethanol extracts from different anatomical parts of 27 plant species (18 families), native to the Pantanal and Cerrado biomes in Midwest Brazil, was assessed for their effect against Aedes aegypti larvae, the vector of dengue, hemorrhagic dengue, Zika and chikungunya fevers. Only the extract obtained from seeds of Guarea kunthiana (Meliaceae) proved active (LC50 = 169.93 μg/mL). A bioassay-guided investigation of this extract led to the isolation and identification of melianodiol, a protolimonoid, as the active constituent (LC50 = 14.44 mg/mL). Meliantriol, which was also obtained from the bioactive fraction, was nevertheless devoid of any larval toxicity, even at the highest concentration tested (LC50 > 100.0 mg/mL). These results indicate that the larvicidal activity of melianodiol stems from the presence of the carbonyl moiety at C-3 in the 21,23-epoxy-21,24,25-trihydroxy-tirucall-7-ene-type skeleton. The structures of both protolimonoids were established on the basis of spectral methods (1H and 13C NMR and MS). This is the first report on the toxicity of melianodiol against Ae. aegypti larvae. Based on the results, melianodiol can be regarded as a potential candidate for use as an ecologically sound biocontrol agent for reducing the larval population of this vector.

Cytotoxicity, nitric oxide and acetylcholinesterase inhibitory activity of three limonoids isolated from Trichilia welwitschii (Meliaceae)

BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC. RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 µg/mL. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin used as reference cytotoxic compounds. Compound (1) (LC50 of 23.55 µg/mL) had good antiproliferative activity against RAW 264.7 cancer cells. At the lowest concentration tested (0.5 µg/mL), compound (2) and (3) released the lowest amount of nitric oxide (2.97 and 2.93 µM, respectively). The three limonoids had anti-AChE activity with IC50 values ranged of 19.13 µg/mL for (1), 34.15 µg/mL for (2) and 45.66 µg/mL for (3), compared to galantamine (IC50 of 8.22 µg/mL) used as positive control. CONCLUSION: The limonoid compounds studied in this work inhibited nitric oxide production in LPS-stimulated macrophages and had anti-AChE activity. Trichilia lactone D5 had potential antiproliferative activity against RAW 264.7 cancer cells. The limonoids had low cytotoxicity towards Vero cells lines. This study provided further examples of the importance of limonoids compounds as potential AChE inhibitors and anti-inflammatory agents targeting the inhibition of NO production.

Cumulative mortality of Aedes aegypti larvae treated with compounds / Mortalidade acumulativa de larvas de Aedes aegypti tratadas com compostos

OBJECTIVE To evaluate the larvicidal activity of Azadirachta indica, Melaleuca alternifolia, carapa guianensis essential oils and fermented extract of Carica papaya against Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae). METHODS The larvicide test was performed in triplicate with 300 larvae for each experimental group using the third larval stage, which were exposed for 24h. The groups were: positive control with industrial larvicide (BTI) in concentrations of 0.37 ppm (PC1) and 0.06 ppm (PC2); treated with compounds of essential oils and fermented extract, 50.0% concentration (G1); treated with compounds of essential oils and fermented extract, 25.0% concentration (G2); treated with compounds of essential oils and fermented extract, 12.5% concentration (G3); and negative control group using water (NC1) and using dimethyl (NC2). The larvae were monitored every 60 min using direct visualization. RESULTS No mortality occurred in experimental groups NC1 and NC2 in the 24h exposure period, whereas there was 100% mortality in the PC1 and PC2 groups compared to NC1 and NC2. Mortality rates of 65.0%, 50.0% and 78.0% were observed in the groups G1, G2 and G3 respectively, compared with NC1 and NC2. CONCLUSIONS The association between three essential oils from Azadirachta indica, Melaleuca alternifolia, Carapa guianensis and fermented extract of Carica papaya was efficient at all concentrations. Therefore, it can be used in Aedes aegypti Liverpool third larvae stage control programs. .

OBJETIVO Avaliar a eficácia de composto de óleos essenciais de Azadirachta indica, Melaleuca alternifolia, Carapa guianensis e extrato fermentado de Carica papaya sobre larvas de Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae). MÉTODOS O ensaio larvicida foi realizado em triplicata com 300 larvas para cada grupo experimental utilizando larvas de terceiro estádio, as quais foram expostas por 24h, em 2013. Os grupos foram: controles positivos com larvicida industrial (BTI) nas concentrações de 0,37 ppm (CP1) e 0,06 ppm (CP2); tratado com composto de óleos essenciais e extrato fermentado na concentração de 50,0% (G1); tratado composto e óleos essenciais e extrato fermentado na concentração de 25,0% (G2); tratado com composto de óleos essenciais e um extrato fermentado na concentração de 12,5% (G3); controle negativo com água (CN1) e controle dimetil sulfóxido (CN2). As larvas foram monitoradas a cada 60 min através de visualização direta. RESULTADOS Larvas dos grupos CN1 e CN2 não tiveram mortalidade durante o período de 24h de exposição, mas os grupos CP1 e CP2 apresentaram taxa de mortalidade de 100% em relação a CN1 e CN2. Os tratamentos G1, G2 e G3 exerceram atividade larvicida de 65,0%, 50,0% e 78,0%, respectivamente, quando comparados a CN1 e CN2. CONCLUSÕES A associação entre os três óleos essenciais de Azadirachta indica, Melaleuca alternifolia, Carapa guianensis e extrato fermentado de Carica papaya foi eficiente em todas as concentrações testadas, podendo ser utilizado no controle de larvas de terceiro estádio de A. aegypti linhagem Liverpool. .

Effect of andiroba oil on periodontitis in Wistar rats

PURPOSE: To evaluate the effects of andiroba oil on the periodontitis in rats. METHODS: The periodontitis was induced by the placement of cotton ligatures around the cervix of the second upper molars on fifteen rats, and waiting fifty days. The animals were randomly distributed into three groups: saline group, andiroba oil group and meloxican group, differentiated by substance used in the treatment of periodontitis. The groups received the respective substance by gavage for seven days, after the periodontitis induced. It was analyzed the score of inflammatory cells and the measurement from the cemento-enamel junction to the bone crest. RESULTS: The andiroba oil group (p=0.008) and meloxican group (p=0.0347) show a less score of inflammatory cells than saline group, however there weren't difference between them (p=0.2754). Regarding the analysis of measurement from the cemento-enamel junction to the bone crest, there was no difference between groups studied (p=0.3451). CONCLUSION: Andiroba oil decreased the quantity of inflammatory cells, however, it didn't have an effect on the measurement of alveolar bone loss, like the treatment with Meloxican®.

Ethnopharmacological evaluation of Trichilia hirta L. as anticancer source in traditional medicine of Santiago de Cuba / Evaluación etnofarmacológica de Trichilia hirta como una fuente anticancer en la medicina tradicional de Santiago de Cuba

Trichilia hirta L. (Meliaceae) is traditionally used as antitumor source in Santiago de Cuba. Therefore, the aim of this study was to document and analyze the traditional medicinal use of this plant by cancer patients in Santiago de Cuba and to evaluate its antiproliferative activity on human normal and cancer cells. Cancer patients consuming Trichilia hirta extracts (Jubabán) were randomly selected and interviewed. The antiproliferative activity of a polysaccharide-rich fraction from leaves was evaluated against normal (MRC-5) and cancer cells (A-549, HeLa and Hep-2) by MTT assay. The study revealed that Trichilia hirta extracts are mainly used as anticancer source (46 percent. Moreover, the majority of cancer patients consuming Trichilia hirta extracts had carcinoma (86 percent). In particular, the most frequent were lung (26 percent) and prostate (18 percent) carcinoma. The majority (90 percent) of patients were consuming the extracts simultaneously, or after the chemotherapy and radiotherapy treatment. The polysaccharide-rich fraction showed antiproliferative activity against human lung cancer cells (A-549) and human cervix carcinoma (HeLa) cancer cells. However, no toxicity was observed in human normal fibroblasts (MRC-5). These results suggest that polysaccharide-rich fraction from Trichilia hirta contribute to the antitumor properties of this specie.

Trichilia hirta L. (Meliaceae) es tradicionalmente usada como recurso antitumoral en Santiago de Cuba. Por lo que, el objetivo de este estudio fue documentar y analizar el uso tradicional de esta planta por pacientes con cáncer en Santiago de Cuba y evaluar su actividad antiproliferativa sobre células humanas normales y tumorales. Pacientes con cáncer consumiendo los extractos de Trichilia hirta (jubabán) fueron aleatoriamente seleccionados y entrevistados. La actividad antiproliferativa de la fracción rica en polisacáridos de hojas fue evaluada en células normales (MRC-5) y en células tumorales (A-549, HeLa y Hep-2) a través del ensayo con MTT. El estudio reveló que los extractos de Trichilia hirta eran usados mayoritariamente como recurso antitumoral (46 por ciento). Además, la mayoría de los pacientes consumiendo extractos de Trichilia hirta presentaron carcinoma (86 por ciento). En particular, los más frecuentes fueron carcinomas de pulmón (26 por ciento) y próstata (18 por ciento). También la mayoría de los pacientes (90 por ciento) consumieron los extractos simultáneamente o después de tratamientos con quimioterapia y radioterapia. La fracción rica en polisacáridos mostró actividad antiproliferativa contra las células de cáncer de pulmón humano (A-549) y carcinoma de cerviz humano (HeLa). Sin embargo, no se observó toxicidad en fibroblastos humanos normales (MRC-5). Estos resultados sugieren que la fracción rica en polisacáridos de hojas de Trichilia hirta contribuye a la actividad antitumoral de esta especie.

Immunorestorative in immunosuppressed Balb/c mice and cytotoxic activity of water extract from Trichilia hirta root / Actividad inmunorestauradora en ratones Balb/c inmunodeprimidos y citotóxica del extracto acuoso de raíz de Trichilia hirta

Patients receiving chemotherapy treatment in Santiago de Cuba traditionally use water extracts from Trichilia hirta roots. The study aim was to evaluate the immunorestorative and cytotoxic activity of water extracts from Trichilia hirta root. Administration of root water extract increased the total and differential leukocyte counts in inmunosupressed Balb/c mice. Thymus weight recovered significantly as well as bone marrow cellularity. Moreover, water extract (125 ug/mL) showed selective cytotoxicity against cancer cells T-47D and SK-mel-3 in comparison with non-cancer cells (Vero). The results indicate that Trichilia hirta has significant immunorestorative effects in vivo and selective cytotoxicity in vitro. Therefore, it might be a promising alternative for cancer therapy.

Pacientes bajo tratamiento quimioterapéutico tradicionalmente usan extractos acuosos de raíz de Trichilia hirta en Santiago de Cuba. El objetivo de este estudio fue evaluar la actividad inmunorestauradora y citotóxica de extractos acuosos de raíz de Trichilia hirta. La administración del extracto acuoso de raíz incrementó los conteos globales y diferenciales de leucocitos en ratones inmunodeprimidos. El peso del timo, así como, la celularidad de la médula ósea se recuperaron significativamente. Además, el extracto acuoso (125 ug/mL) mostró citotoxicidad selectiva contra las células tumorales T-47D y SK-mel-3 en comparación con la línea no tumoral (Vero). Los resultados indican que Trichilia hirta posee significativos efectos inmunorestauradores in vivo y citotoxicidad selectiva, por lo cual podría ser una promisoria alternativa para la terapia del cáncer.

Efeito de extratos orgânicos de Meliaceae sobre Bemisia tabaci (Gennadius) biótipo B em tomateiro / Effect of organic extracts from Meliaceae on Bemisia tabaci (Gennadius) B biotype in tomato plants

Avaliou-se o efeito de extratos orgânicos de Azadirachta indica. Melia azedarach. Toona ciliata e Trichilia pallida (Rutales: Meliaceae) sobre ovos e ninfas de Bemisia tabaci (Hemiptera: Aleyrodidae) biótipo B. Folhas e ramos foram secos, moídos e submetidos à extração (Soxhlet) em diclorometano e em etanol. Visando estabelecer uma concentração adequada para os estudos subsequentes, foram utilizados ovos oriundos de 40 adultos que foram mantidos por 24h em folíolos de tomateiro em gaiolas de voil. Após 9 dias, contou-se o número de ninfas e pulverizou-se o extrato de folhas de .. pallida em diclorometano em diferentes concentrações (0,32; 0,56; 1,0; 1,8 e 3,2%), além de água e acetona (controles). A mortalidade ninfal foi avaliada após sete dias. O experimento foi conduzido em delineamento inteiramente aleatorizado, com sete tratamentos e quatro repetições. Posteriormente, extratos em diclorometano e em etanol foram pulverizados sobre ovos na concentração selecionada (0,56%), em experimentos distintos para cada solvente. Após nove dias, avaliou-se a mortalidade dos ovos e fez-se nova pulverização, avaliando-se, após sete dias, a mortalidade ninfal. Os experimentos foram conduzidos em delineamento com blocos aleatorizados (10 tratamentos e quatro repetições). Os dados foram submetidos à análise de regressão, análise de variância e comparados pelo teste de Tukey. Observou-se maior rendimento dos extratos em etanol em relação aos extratos em diclorometano. Os extratos em diclorometano de folhas e de ramos das meliáceas afetaram significativamente a sobrevivência de ovos e ninfas da mosca-branca. Os extratos em etanol afetaram a fase de ninfa, mas não apresentaram atividade ovicida.

The effect of organic extracts from Azadirachta indica. Melia azedarach. Toona ciliata and Trichilia pallida (Rutales: Meliaceae) on eggs and nymphs of Bemisia tabaci (Hemiptera: Aleyrodidae) B biotype was assessed. Leaves and branches were dried, ground and submitted to extraction (Soxhlet) with dichloromethane and ethanol. To establish an adequate concentration for the subsequent tests, eggs were used originating from 40 adults maintained for 24h on tomato leaves in voile cages. After 9 days, the number of nymphs was determined and the dichloromethane extract from T. pallida leaves was sprayed at different concentrations (0.32, 0.56, 1.0, 1.8 and 3.2%); water and acetone were also used (controls). The nymphal mortality was evaluated after 7 days. An entirely randomized design was used, with 7 treatments and 4 replications. Afterwards, dichloromethane and ethanol extracts were sprayed on eggs at the selected concentration (0.56%), in different experiments. After 9 days, the egg mortality was evaluated and a new spraying was conducted to assess the nymphal mortality after 7 days. These experiments were conducted in a randomized block designed (10 treatments and 4 replications). The data was submitted to regression analysis, analysis of variance and compared by the Tukey test. A higher yield was observed for the ethanol extracts in relation to the dichloromethane extracts. The dichloromethane extracts from leaves and branches from meliaceas significantly affected the survival of the whitefly eggs and nymphs. The ethanol extracts affected the nymphal phase, but did not present ovicidal activity.

Plant growth inhibitory activity of N, N´-di-(4-R-phenyl)-alkanediamides / Actividad inhibitoria de crecimiento de plantas de N, N’-di-(4-R-phenyl)-alcanodiamidas

Diamides are a class of metabolites that occurring in some Meliaceae plants, in Aglaia spp for example, with an ample body of biological activities, being insecticidal and herbicidal two of the most important. In our program of search for botanical pesticides, a series of N,N´-di-(4-R-phenyl)-alkanediamides was evaluated for its herbicidal activity. Many of the analogues tested exhibited moderate to good herbicidal activity both pre-emergence and post-emergence and have been found to inhibit energetic metabolism of pre-emergence weeds. The structure-activity relationships were probed by substitution on the benzene ring. Among the variations investigated, it was found that maximal herbicidal activity was obtained by substitution of –F, -CN and -Br at the aromatic portion and by n=2 of the aliphatic long chain. This last number of carbons (n=2) substitution was the key for the inhibitory activity.

Diamidas son una clase de metabolitos que estan presentes en plantas perteneciente a la familia de la Meliaceas, en Aglaia por ejemplo, poseen un amplio cuerpo de actividades biologicas, siendo la insecticida y la herbicida dos de las mas importantes. En nuestro programa para la busqueda de pesticidas botanicos, una serie de N,N’-di-(4-R-phenyl)-alkanodiamidas se evaluo para su actividad herbicida. Muchos de los analogos exhibieron desde buenas a moderadas actividades, tanto como pre-emergentes como post-emergentes y ademas se encontro que inhiben el metabolismo pre-emergente energetico de malezas. La relacion estructura-actividad fue probada por sustitución sobre al anillo aromatico. Entre las variaciones investigadas, se encontro que la maxima actividad herbicida se obtuvo por sustitución de F, CN y Br en la porcion aromatica y por n=2 del largo de la cadena alifatica. Este ultimo numero de carbonos de sustitución (n=2) fue clave para la actividad inhibitoria.

Bioatividade de moléculas isoladas de Trichilia pallida Swartz (Meliaceae) sobre Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) / Bioactivity of Trichilia pallida Swartz (Meliaceae) derived molecules on Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

A partir do extrato em diclorometano (DIC) de folhas e frutos de Trichilia pallida Swartz, objetivou-se fazer o isolamento e identificação de substâncias com atividade inseticida sobre a traça-do-tomateiro, Tuta absoluta (Meyrick). Do extrato em DIC de folhas de T. pallida foram isolados os triterpenos 24-metilenocicloarta-3β-ol (TRIT-1), 24-metilenocicloarta-3β-26-diol (TRIT-2) e cicloarta-23-eno-3β,25-diol (TRIT-3), os esteróides 24-metileno-3,22-diidroxicolesterol (EST-1), 24-metilenocolesterol (EST-2) e 24-metileno-3β,4β,22-triidroxicolesterol (EST-3), além do limonóide gedunina (LIM) obtido de frutos da planta. As substâncias foram dissolvidas em acetona e pulverizadas a 0,1% em folíolos de tomateiro infestados com lagartas recém-eclodidas. Foram avaliadas a mortalidade de lagartas aos quinto e nono dias após a infestação, duração e sobrevivência das fases de larva e pupa, peso de pupas e porcentagem de adultos deformados. TRIT-1, EST-1 e LIM foram as moléculas que apresentaram maior atividade sobre T. absoluta, alongando o desenvolvimento larval e reduzindo a sobrevivência dessa fase.

Dichloromethane (DIC) leaf and fruit extracts of Trichiliapallida Swartz were obtained for the isolation and identification of molecules with insecticidal activity against the tomato leafminer, Tuta absoluta (Meyrick). DIC leaf extracts of T. pallida yielded six compounds, the triterpenes 24-methylenecycloarta-3β-ol (TRIT-1), 24-methylenecycloarta-3β-26-diol (TRIT-2) and cycloarta-23-eno-3β,25-diol (TRIT-3), the sterols 24-methylene-3,22-dihydroxycholesterol (EST-1), 24-methylenecholesterol (EST-2) and 24-methylene-3β,4β,22-trihydroxycholesterol (EST-3), while the fruit extract yielded the limonoid gedunine (LIM). These molecules were dissolved in acetone and sprayed at 0.1% on tomato leaflets infested with newly-hatched larvae. Larval mortality at day 5 and 9 after infestation, larval and pupal developmental time and survival, pupal weight and adult malformation were evaluated. TRIT-1, EST-1 and LIM were the most effective against T. absoluta due to larval development arrestment and reduced larval survivorship.

Virucidal activity presence in Trichilia glabra leaves / Presencia de actividad antiviral en hojas de Trichilia glabra

Different immunomodulatory activities present in Trichilia glabra (TG) leaf extracts have already been described. Particularly, chloroform-methanol extracts were responsible for an in-vivo anti-inflammatory effect. The effect of such extracts on the infectivity of enveloped and naked viruses were investigated. Methanolic fraction extracts were active against herpes simplex virus type 1 (HSV-1) and vesicular stomatitis virus (VSV), while no activity against poliovirus type 3 was observed. VSV was slightly more affected than HSV-1: 2.8 log10 reduction in VSV titer against 2.4 log10reduction in HSV-1 titer when 0.25 mg/ml F2 fraction was tested and a reduction of 2.7 log10in VSV virus titer and of 1.5 log10in HSV-1 virus titer was observed when 0.25 mg/ml F3 fraction was tested. Results obtained in this work suggest a potential pharmaceutical use of TG extract components.

Previamente se han descripto distintas actividades inmunomoduladoras, presentes en extractos de hojas de Trichilia glabra (TG). En particular, se ha demostrado una actividad antiinflamatoria presente en extractos metanólicos. En este trabajo se investigó la actividad virucida de dichos extractos sobre virus envueltos y desnudos. Distintos extractos metanólicos han inactivado en forma moderada los virus herpes simplex tipo 1 (HSV-1) y el virus de la estomatitis vesicular (VSV), mientras no evidenciaron actividad sobre poliovirus tipo 3. VSV resultó algo mas afectado que HSV-1: se observó una reducción en el título viral de 2,8 log10para VSV y de 2,4 log10para HSV-1 cuando se uso una concentración de 0,25 mg/ml de la fracción F2 y una reducción de 2,7 log10para VSV y de 1,5 log 10para HSV-1 cuando se usó una concentración de 0,25 mg/ml de la fracción F3. Los resultados obtenidos en este trabajo, sugieren un potencial uso farmacéutico de los componentes presentes en los extractos de TG.